Download Animal Cell Technology: Basic & Applied Aspects: Proceedings by Mitsuo Satoh, Shigeru Iida (auth.), Sanetaka Shirahata, Koji PDF

By Mitsuo Satoh, Shigeru Iida (auth.), Sanetaka Shirahata, Koji Ikura, Masaya Nagao, Akira Ichikawa, Kiichiro Teruya (eds.)

Animal phone expertise is a growing to be self-discipline of telephone biology which goals not just to appreciate buildings, capabilities and behaviors of differentiated animal cells, but in addition to examine their skills for use for business and scientific reasons. The target of animal cellphone know-how comprises the clonal enlargement of differentiated cells, the optimization in their tradition stipulations, modulation in their skill to provide proteins of clinical and pharmaceutical importantance, and the applying of animal cells to gene remedy, man made organs and the creation of sensible meals. This quantity supplies the readers an entire evaluation of the current cutting-edge and should be necessary for these operating in both educational environments or within the biotechnology and pharmaceutical sectors, rather telephone biologists, biochemists, molecular biologists, immunologists, biochemical engineers and all different disciplines relating to animal telephone culture.

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Extra resources for Animal Cell Technology: Basic & Applied Aspects: Proceedings of the 19th Annual Meeting of the Japanese Association for Animal Cell Technology (JAACT), Kyoto, Japan, September 25-28, 2006

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05) higher when there were no growth factors at all. Keywords Serum • HEK 293 • adenovirus • growth factors 1 Introduction The HEK 293 cell line came about from Kidney cells derived from an aborted human foetus and produced by transfection of sheared Adenovirus human type 5 DNA so they contain the E1 gene needed for viral replication [1]. These cells are A. Buckler Department of Chemical Engineering, University of Birmingham, Birmingham, B15 2TT, UK M. Al-Rubeai School of Chemical and Bioprocess Engineering, University College Dublin, Belfield, Dublin 4, Ireland K.

Biophys. Res. , 315, 132–138 (2004). Effects of Serum and Growth Factors on HEK 293 Proliferation and Adenovirus Productivity Angela Buckler and Mohamed Al-Rubeai Abstract The effect of serum on cell growth and virus production in both basal and serum free media was examined in Human Embryonic Kidney (HEK) 293. A 40-fold decrease in virus titre was observed in Dulbecco’s Modified Eagles Medium (DMEM) cultures supplemented with 10% FBS when compared to 1% FBS. However, a 90-fold increase in virus titre was observed in DMEM supplemented with 1% FBS as compared with EX-CELL™ 293 serum free culture.

Figure 2 shows that activity of caspase3/7, an executioner for inducing apoptosis, was decreased by sericin treatment. In contrast, caspase9 activity Effects of Sericin on Promoting Proliferation and Inhibiting Apoptosis 35 was not decreased by sericin (data not shown). These results suggest that sericin suppressed apoptosis via suppression of caspase3/7. 2 Detection of Genes Involved in the Mode of Action of Sericin To analyze the mode of action of sericin in detail, we examined the gene expression pattern from 2E3-O cells by utilizing cDNA microarrays.

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